
| Polynesia, the parrot | RONNIE LE DREW |
| Dr. John Dolittle, a country doctor | RUSS ABBOT |
| Jip | MATTHEW LYONS |
| Tommy Stubbins, a ten-year-old local boy | MATTHEW BROWN/JONATHAN BOURKE/SAM WILMOTT |
| Matthew Mugg, the Cats’ Fish Man | DAVID GANLY |
| Chee-Chee, the chimpanzee | CLARE GOODMAN |
| General Bellowes, a local landowner | GEORGE LITTLE |
| Emma Fairfax, his niece | LIZA PULMAN |
| Albert Blossom, a circus owner | JOHN RAWNSLEY |
| Gertie Blossom, his wife | KATHRYN OTTO |
| Straight Arrow, Dolittle's colleague on Sea-Star-Island | SAM MANCUSO |
| The Voice of Polynesia | JULIE ANDREWS |
| Dab-Dab | IESTYN EVANS |
| Gub-Gub | ANDY HEATH |
| The Goat | SAM MASON |
| Toggle | JOHN GINLEY |
| Pushmi-Pullyu | KAREN FOLEY/VICTORIA MILLEN |
| Sophie | SARAH BURGESS |
| Rufus | ROBERT SKIDMORE |
| Animals/Puppeteers/ Residents of Sea-Star-Island/ Albert Blossom Circus Troupe |